thr77 for Dummies
thr77 for Dummies
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in endorsing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been explained. Two extra splice variants were being described but specific nucleotide sequence details is just not nevertheless readily available.
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Blocking peptides are peptides that bind specially to the target antibody and block antibody binding. These peptide typically incorporates the epitope recognized with the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the best possible results are...
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This guidebook outlines more attainable optimization of mobile and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is particular.
Goal Data Cyclin E belongs into the highly conserved cyclin loved ones, whose users are characterized by a remarkable periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates for the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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